A woman from South Australia is among the injured in the London terror attack, parliament has been told.
Five people were killed, including the assailant, and 40 others were injured in what UK Prime Minister Theresa May has condemned as a "sick and depraved terrorist attack."
Inviting British High Commissioner Menna Rawlings into the parliamentary chamber on Thursday to listen to condolence speeches, Prime Minister Malcolm Turnbull revealed an Australian permanent resident had been injured and was receiving treatment in hospital.
Mr Turnbull also praised the "act of heroism" of the British MP Tobias Ellwood who tried to resuscitate an injured police officer who subsequently died outside the British parliament.
The MP's brother Jon died in the Bali bombing in 2002.
"It was an attack on parliaments, freedom and democracy everywhere in the world," Mr Turnbull said.
"Westminster is rightly known as the mother of parliaments."
Australia stood in "heartfelt solidarity" with Britain.
Mr Turnbull said Australians should be reassured agencies are working "relentlessly and tirelessly to keep our people safe".
"We will never let the terrorists win. Not on the battlefield, not here at home, we will never change the way we live."
Labor leader Bill Shorten told parliament the opposition shared the government's commitment to security.
"We say to those who seek to spread fear, who shed blood to spread fear, you will not succeed," Mr Shorten said.
"You will not divide a people or a world determined and too strong to defeat your ideology of evil."
Justice Minister Michael Keenan said Australia's Parliament House remained safe.
Mr Keenan said $114 million had been spent on the Australian Federal Police presence in the parliamentary precinct in Canberra, including on long-arm rifles and bomb dogs.
A further $126 million had been spent on physical security in the precinct.
AFP officers had been equipped with stab-proof vests as part of a $180 million program to secure them and their office buildings.